ABSTRACT
The aim of this study was to look for the chemical constituents from the rhizomes of Dryopteris sublaeta. The fresh plant was extracted twice with boiling water, the extract was concentrated to small volume under reduced pressure at 50 degrees C. The concentrated material was partitioned with ether, ethyl acetate and n-butanol. The fraction of ethyl acetate was repeatedly chromatographied over silica gel and Sephadex LH-20 columns. Structures of pure compounds were established on the basis of their physiochemical and spectral data. Nine compounds were obtained and identified as sublaetentin A (1), sublaetentin B (2), sublaetentin C (3), sublaetentin D (4), matteuorienate A (5), matteuorienate C (6), arbutin (7), 3-methoxy-4-hydroxyphenyl-1-O-beta-D-glucopyranoside (8) and 3,4-dimethoxyphenyl-1-O-beta-D-glucopyranoside (9). Compounds 1 - 4 are new compounds, the others were isolated from this plant for the first time.
Subject(s)
Arbutin , Chemistry , Dryopteris , Chemistry , Flavanones , Chemistry , Flavonoids , Chemistry , Glucosides , Chemistry , Glycosides , Chemistry , Molecular Structure , Plant Extracts , Chemistry , Plants, Medicinal , Chemistry , Rhizome , ChemistryABSTRACT
<p><b>AIM</b>To study the chemical constituents of Dryopteris sublaeta Ching et Hsu.</p><p><b>METHODS</b>Fresh plant of Dryopteris sublaeta Ching et Hsu was extracted twice with boiling water, the extract was concentrated to small volume under reduced pressure at 50 degrees C. The concentrated material was partitioned with ether, ethyl acetate, and n-butanol. The fraction of ether extract was chromatographed over silica gel column. The compounds were identified on the basis of their physiochemical and spectral data.</p><p><b>RESULTS</b>Four compounds were obtained and identified as 2 (S)-5, 7, 3'-trihydroxy-6, 8-dimethyl-5'-methoxyflavanone (1), matteucinol (2), desmethoxymatteucinol (3) and 5, 7, 2'-trihydroxy-6, 8-dimethylflavanone (4).</p><p><b>CONCLUSION</b>Compound 1 is a new one, the others were isolated from Dryopteris for the first time.</p>
Subject(s)
Chromones , Chemistry , Dryopteris , Chemistry , Flavanones , Chemistry , Molecular Conformation , Molecular Structure , Plants, Medicinal , ChemistryABSTRACT
<p><b>AIM</b>To study the chemical constituents of Dryopteris sublaeta Ching et Hsu.</p><p><b>METHODS</b>Fresh plant of Dryopteris sublaeta ching et hsu was extracted twice with boiling water, concentrated to small volume under reduced pressure at 50 degrees C. The concentrated material was partitioned with ether, ethyl acetate, and n-butanol. The fraction of ethyl acetate extract was chromatographed over macroporous adsorption resin (Diaion HP-20) eluted with a mixture of H2O and MeOH in increasing MeOH content. Their fractions from resin were repeatedly chromatographed over Toyopearl HW-40, Sephadex LH-20 and silica gel column chromatography. The compounds were identified on the basis of their physiochemical and spectral data.</p><p><b>RESULTS</b>Four compounds were obtained and identified as 3,5-dihydroxy-stilbene-3-O-neohesperidoside (1), 3,5-dihydroxy-stilbene-3-O-beta-D-glucoside (2), polydotin peceid (3) and 3,5,4'-trihydroxy-bibenzyl-3-O-beta-D-glucoside (4).</p><p><b>CONCLUSION</b>Compound 1 is a new compound, the others were isolated from Dryopteris for the first time.</p>